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M94A0250.TXT
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1994-10-08
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Document 0250
DOCN M94A0250
TI Conditional regulatory elements of human immunodeficiency virus type 2
long terminal repeat.
DT 9412
AU Arya SK; Mohr JR; Laboratory of Tumor Cell Biology, National Cancer
Institute,; National Institutes of Health, Bethesda, Maryland 20892.
SO J Gen Virol. 1994 Sep;75 ( Pt 9):2253-60. Unique Identifier : AIDSLINE
MED/94358723
AB Mutational analysis of the human immunodeficiency virus type 2 (HIV-2)
long terminal repeat (LTR) revealed a novel cis-acting positive and a
negative regulatory element in the U3 region, located upstream of the
enhancer-promoter region. These elements acted in a cell type-specific
manner, being most active in human lymphocytic CEM cells, more active in
Jurkat cells than in human monocytic U937 cells and least active in
epithelioid HeLa cells. The down-modulatory effect of the negative
regulatory element was abolished by HIV-2 Tat, suggesting the
involvement of upstream DNA elements in optimal Tat-mediated
trans-activation. The sequence elements that respond to T cell
activation signals were also located in the upstream U3 region. Notably,
the magnitude of the effect of the upstream regulatory elements depended
on the basal activity of the LTR, which was also cell type-dependent.
This emphasizes the importance of the cell-specific transcriptional
factors and other effectors in regulating HIV gene expression. These
observations may be relevant to the cell type-specific restriction of
virus replication in vivo.
DE Base Sequence Cell Line Chloramphenicol Acetyltransferase/BIOSYNTHESIS
Cloning, Molecular Comparative Study DNA Mutational Analysis DNA,
Viral/CHEMISTRY/GENETICS Enhancer Elements (Genetics) Gene Expression
Hela Cells Human *HIV Long Terminal Repeat HIV-2/*GENETICS
Lymphocytes Molecular Sequence Data Mutagenesis Promoter Regions
(Genetics) Proviruses/GENETICS *Regulatory Sequences, Nucleic Acid
Support, U.S. Gov't, P.H.S. Transfection JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).